CONTAMINATION OF FETAL BOVINE SERA BY TRANSFORMED TUBERCULOSIS MYCOBACTERIA-Reference-Cited by-同舟云学术

CONTAMINATION OF FETAL BOVINE SERA BY TRANSFORMED TUBERCULOSIS MYCOBACTERIA

Published:2022-12-14 Issue:2 Volume: Page:59-69
ISSN:2224-1647
Container-title:Ecology and Animal World
language:
Short-container-title:Èkol. životn. mir

Author:

Lysenko A. P.,Kuchvalski M. V.,Pritychenko A. N.,Krasnikova E. L.,Anikevich N. Y.

Abstract

In commercial lots of fetal bovine serum (FBS), the tuberculosis mycobacteria (MTB) genome was detected and with cell wall deficient (CWD) MTB were isolated. It is clearly associated with the widespread latent tuberculosis infection that is not detected by tuberculin test and traditional bacteriological methods. The seriousness of the prob-lem of such contamination was confirmed by the isolation of CWD MTB from live and inactivated virus vaccines that were obtained using FBS. Isolates from FBS and vaccines demonstrated adaptive properties inherent in CWD MTB, withstood extreme heating and chemical action. It is indicated that it is necessary to control FBS for the presence of the MTB genome and by inoculating on the special nutrient medium after incubation in the growth stimulant due to the risk of neoplastic processes increased by contaminated vaccines, and due to induced reactions to bovine tuberculin in certain cases.

Publisher

National Academy of Sciences of Belarus

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