Cloning a Gene Coding for Norflurazon Resistance in Cyanobacteria-Reference-Cited by-同舟云学术

Cloning a Gene Coding for Norflurazon Resistance in Cyanobacteria

Published:1990-05-01 Issue:5 Volume:45 Page:482-486
ISSN:1865-7125
Container-title:Zeitschrift für Naturforschung C
language:en
Short-container-title:

Author:

Chamovitz Daniel¹,Pecker Iris¹,Sandmann Gerhard²,Böger Peter²,Hirschberg Joseph¹

Affiliation:

1. Department of Genetics, The Hebrew University of Jerusalem, Jerusalem 91904, Israel

2. Lehrstuhl für Physiologie und Biochemie der Pflanzen. Universität Konstanz, D-7750 Konstanz, Bundesrepublik Deutschland

Abstract

The herbicide norflurazon inhibits carotene biosynthesis in photosynthetic organisms by blocking the enzyme phytoene dehydrogenase (= phytoene desaturase). We have isolated norflurazon-resistant mutants of the cyanobacterium Synechococcus PCC7942. The herbicide- resistance gene from the mutant NFZ4 has been cloned by genetic complementation of the resistance trait in wild type cells. The experiment described here illustrates the usefulness of employing cyanobacteria to clone herbicide-resistance genes in a quick and simple way.

Publisher

Walter de Gruyter GmbH

Subject

General Biochemistry, Genetics and Molecular Biology

Link

https://www.degruyter.com/document/doi/10.1515/znc-1990-0531/pdf

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