„Disc-Elektrophorese“ von löslichen Pflanzenproteinen

Abstract

The soluble proteins from tissues of chicory roots and pea seedlings were analysed by disc electrophoresis. In extracts of chicory root tissue 19 proteins were found by staining with amido-black, also up to 10 esterases, 4 to 5 phosphatases, 1 to 2 glutamic dehydrogenases (GDH) and one leucinaminopeptidase. The amount of the particular proteins and the activities of the main esterases and the GDH could be determined quantitatively. Reproducible separations of the proteins from chicory root and pea seedlings could be obtained only when the extraction medium contained mercaptoethanol (MA) besides polyvinylpyrrolidone or polyethylenglykol. While chicory extracts gave good separations without further treatments, extracts of pea tissue have to be dialysed in the presence of MA to split all existing protein complexes. It then became evident that pherograms of pea root tissues of different ages (in a distance of 1 to 6 mm from the apex) are nearly identical concerning the non-specific proteins stained with amido-black. Also the zymograms of malate dehydrogenase are identical for all root tissues. In contrast, some peroxidases appear only in older root cells.