Blood indices and circulating tumor cells for predicting metastasis of hepatocellular carcinoma after liver transplantation
Author:
Zhuang Li1, Liu Xiang-Yan1, Zhu Heng-Kai1, Wang Zhuo-Yi1, Zhang Wu1, Jiang Guo-Ping1, Zheng Shu-Sen1
Affiliation:
1. Department of Hepatobiliary and Pancreatic Surgery , Shulan (Hangzhou) Hospital Affiliated to Zhejiang Shuren University Shulan International Medical College , Hangzhou , P.R. China
Abstract
Abstract
Objectives
Liver transplantation (LT) can benefit the long-term survival of hepatocellular carcinoma (HCC) patients. We hypothesized that circulating tumor cell (CTC) levels and subtypes are intimately associated with metastasis status of HCC patients. This study was designed to test that compositive hematological indices including CTC can provide a prediction of post-LT metastasis.
Methods
Between 2017 and 2018, 37 HCC patients within Hangzhou criteria receiving LT were included for analysis. The 24-month follow-up was mainly conducted by outpatient and telephone. Blood samples were collected, and hematological indices were examined. The outcomes such as PFS, recurrence, metastasis, location of recurrence/metastasis, and number of metastases were recorded.
Results
The follow-up analysis showed that microvascular invasion (MVI) classification at the baseline is associated with metastasis. Next, α-fetoprotein (AFP) level was another useful indicator of postoperative metastasis, especially at the third or fourth month; the protein induced by vitamin K absence or antagonist-II (PIVKA-II) level three months after LT was significantly higher for those who had later metastasis. The mesenchymal CTC level at the 45th day was increased for in the metastasis group. Using two-ends Logistic regression, the calculated value MP (metastasis predictor, by above factors). Had an AUC of 0.858 in the ROC curve, with a cutoff value of 0.328.
Conclusions
In conclusion, microvascular invasion, AFP level at the third or fourth month, PIVKA-II level at the third month, and mesenchymal CTC level at day 45 were associated with post-LT metastasis. Using Logistic regression based on above variables, the two-year metastasis can be predicted with satisfactory sensitivity and accuracy.
Publisher
Walter de Gruyter GmbH
Subject
Biochemistry (medical),Medical Laboratory Technology,Clinical Biochemistry
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