Affiliation:
1. Department of Oncology and Hematology , Marienhospital Stuttgart , Stuttgart , Germany
2. Department of Laboratory Medicine , Marienhospital Stuttgart , Stuttgart , Germany
3. Medizinische Fakultät Mannheim , Ruprecht Karls Universität , Mannheim , Germany
Abstract
Abstract
Objectives
Diagnosing disturbances in iron metabolism can be challenging when accompanied by inflammation. New diagnostic tools such as the “Thomas-plot” (TP) (relation of soluble transferrin receptor [sTfR]/log ferritin to reticulocyte hemoglobin content [RET-He]) were established to improve classification of anemias. Aim of this retrospective study was to assess the added diagnostic value of the TP in anemia work up.
Methods
Patients from December 2016 to September 2018 with a complete blood count, iron status, RET-He and sTfR were manually classified into the four quadrants of the TP on basis of conventional iron markers. Manual and algorithm-based classifications were compared using cross tabulations, Box–Whisker-Plots as well as Receiver-Operating-Characteristics (ROC) to calculate the diagnostic accuracy using Area under the Curve (AUC) analysis.
Results
A total of 3,745 patients with a conventional iron status, including 1,721 TPs, could be evaluated. In 70% of the cases the manual classification was identical to the TP, in 10% it was deviant. 20% could not clearly be classified, mostly due to inflammatory conditions. In the absence of an inflammatory condition, ferritin was a reliable parameter to define iron deficiency (ID) (AUC 0.958). In the presence of inflammation, the significance of the ferritin index (AUC 0.917) and of the RET-He (AUC 0.957) increased.
Conclusions
The TP can be useful for narrowing down the causes of anemia in complex cases. Further studies with focus on special patient groups, e.g., oncological or rheumatic patients, are desirable.
Subject
Biochemistry, medical,Clinical Biochemistry,Discrete Mathematics and Combinatorics
Reference34 articles.
1. Onkopedia. Onkopedia leitlinien. Eisenmangel und Eisenmangelanämie 2018. Available at: https://www.onkopedia.com/de/onkopedia/guidelines/eisenmangel-und-eisenmangelanaemie/@@view/html/index.html. [Accessed 1 Sep 2019].
2. Camaschella, C. Iron-deficiency anemia. N Engl J Med 2015;372:1832–43. https://doi.org/10.1056/nejmra1401038.
3. Weiss, G, Goodnough, LT. Anemia of chronic disease. N Engl J Med 2005;352:1011–23. https://doi.org/10.1056/nejmra041809.
4. Ganz, T, Nemeth, E. Hepcidin and iron homeostasis. Biochim Biophys Acta 2012;1823:1434–43. https://doi.org/10.1016/j.bbamcr.2012.01.014.
5. La Ferla, K, Reimann, C, Jelkmann, W, Hellwig-Burgel, T. Inhibition of erythropoietin gene expression signaling involves the transcription factors gata-2 and nf-kappab. FASEB J 2002;16:1811–3. https://doi.org/10.1096/fj.02-0168fje.
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