Evaluation of the compatibility of Phoenix 100 and Microflex LT MALDI-TOF MS systems in the identification of routinely isolated microorganisms in the clinic microbiology laboratory

Author:

Celik Cem,Uysal Elif Bilge,Tutar Uğur,Erturk Rahsan,Bakici Mustafa Zahir,Gozel Mustafa Gokhan

Abstract

AbstractMatrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDI-TOF MS) is a quick, reliable, and efficient system for identifying microorganisms. Many centers that use the Phoenix 100 system today may adopt a MALDI-TOF MS system in the future. Our laboratory recently undertook this pivot. The present study evaluates the reproducibility of species identifications made by the Phoenix 100 and MALDI-TOF MS systems, during a period of transitioning laboratory instrumentation.Eight hundred and twelve microbial isolates, from aerobic cultures of different clinical samples, were identified simultaneously with Phoenix 100 (Becton Dickinson, Sparks, MD, USA) and a Microflex LT MALDI-TOF MS (Bruker Daltonics, Bremen, Germany) devices.Both systems made identical species assignments for 98.9%, 92.1%, 95.1%, and 93.1% of Gram-negative isolates, catalase-positive Gram-positive cocci isolates, catalase-negative Gram-positive cocci isolates, andIdentifications made by two instruments commonly used in microbiology laboratories, the Phoenix 100 and the Microflex LT MALDI-TOF MS, are highly consistent.

Publisher

Walter de Gruyter GmbH

Subject

Biochemistry (medical),Medical Laboratory Technology,Clinical Biochemistry

Reference56 articles.

1. Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry for species identification of Nonfermenting Gram-Negative Bacilli;J Microbiol Methods,2015

2. Advances in mass spectrometry for the identification of pathogens;Mass Spectrom Rev,2011

3. Comparison of “matrix-assisted laser desorption ionization- time of flight mass spectrometry” plus chromogenic media with Phoenix automated system in the rapid diagnosis of Salmonella species;Kafkas Univ Vet Fak Derg,2013

4. MALDI-TOF MS: an upcoming tool for rapid detection of antibiotic resistance in microorganisms;Proteomics Clin Appl,2013

5. Maldi –tof mass spectrometry for microorganism identification;Clin Lab Med,2013

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