Effects of sample handling on the stability of interleukin-6 in patients with breast neoplasms-Reference-Cited by-同舟云学术

Effects of sample handling on the stability of interleukin-6 in patients with breast neoplasms

Published:2022-03-01 Issue:2 Volume:46 Page:115-119
ISSN:2567-9449
Container-title:Journal of Laboratory Medicine
language:en
Short-container-title:

Author:

Wang Yunxiu¹,Wang Baiye²,She Lanhui³,Wang Jijuan⁴,Chen Yingting¹,Huang Di¹,Han Guang¹,Lu Miaolian¹,Wu Xiaobin¹,Wan Zemin¹,He Min¹,Ke Peifeng¹,Huang Xianzhang¹,Liu Hongcan¹

Affiliation:

1. Department of Laboratory Medicine , The Second Affiliated Hospital of Guangzhou University of Chinese Medicine , Guangzhou , P.R. China

2. Department of Radiology , Zhujiang Hospital Southern Medical University , Guangzhou , Guangdong , P.R. China

3. Department of Infectious Diseases , Guangzhou Women and Children’s Medical Center , Guangzhou , Guangdong , P.R. China

4. Department of Internal Medicine , The Second Affiliated Hospital of Guangzhou University of Chinese Medicine , Guangzhou , P.R. China

Abstract

Abstract Objectives To investigate the influence of preservation methods and processes on the plasma interleukin-6 (IL-6) stability. Methods Lithium-heparin plasma was collected from female patients: 32 female patients with invasive breast neoplasms and 20 healthy females. Each sample was divided into three tubes. Samples were stored at different temperatures or at different times. The concentration of IL-6 was detected. Results IL-6 levels in patients were not altered significantly compared to the control group after storage at 4 °C or 25 °C for 12 h. However, IL-6 levels were significantly higher compared to controls (p<0.05) after storage at 25 °C for 48 h. IL-6 levels in patients with breast neoplasms were significantly higher compared to the control group (p<0.05) when stored at 4 °C after 12 h. IL-6 levels in patients with breast neoplasm increased more than 10-folds compared to the control group after only 2 h storage at 25 °C. Conclusions Concentrations of IL-6 in breast neoplasms samples significantly change under different storage conditions. Pretreatment needs to be standardized for blood sample handling procedure. Comparison of different storage conditions of IL-6 levels may not be reliable.

Funder

China Postdoctoral Science Foundation

Doctoral Initiating Foundation of Guangdong for Natural Sciences

Publisher

Walter de Gruyter GmbH

Subject

Biochemistry (medical),Clinical Biochemistry,Discrete Mathematics and Combinatorics

Link

https://www.degruyter.com/document/doi/10.1515/labmed-2020-0155/pdf

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