Evaluation of red blood cell parameters provided by the UF-5000 urine auto-analyzer in patients with glomerulonephritis
Author:
Mizuno Genki1, Hoshi Masato12ORCID, Nakamoto Kentaro13, Sakurai Masayo1, Nagashima Kazuko1, Fujita Takashi1, Ito Hiroyasu1, Hata Tadayoshi1
Affiliation:
1. Department of Clinical Laboratory , Fujita Health University Hospital , Kutsukakecho , Toyoake , Aichi , Japan 2. Department of Biochemical and Analytical Science , Fujita Health University , Kutsukakecho , Toyoake , Aichi , Japan 3. Department of Disease Control and Prevention , Fujita Health University , Kutsukakecho , Toyoake , Aichi , Japan
Abstract
Abstract
Objectives
The microscopic examination of hematuria, a cardinal symptom of glomerulonephritis (GN), is time-consuming and labor-intensive. As an alternative, the fully automated urine particle analyzer UF-5000 can interpret the morphological information of the glomerular red blood cells (RBCs) using parameters such as UF-5000 small RBCs (UF-%sRBCs) and Lysed-RBCs.
Methods
Hematuria samples from 203 patients were analyzed using the UF-5000 and blood and urine chemistries to determine the cut-off values of RBC parameters for GN and non-glomerulonephritis (NGN) classification and confirm their sensitivity to the IgA nephropathy and non-IgA nephropathy groups.
Results
The UF-%sRBCs and Lysed-RBCs values differed significantly between the GN and NGN groups. The cut-off value of UF-%sRBCs was >56.8% (area under the curve, 0.649; sensitivity, 94.1%; specificity, 38.1%; positive predictive value, 68.3%; and negative predictive value, 82.1%), while that for Lysed-RBC was >4.6/μL (area under the curve, 0.708; sensitivity, 82.4%; specificity, 56.0%; positive predictive value, 72.6%; and negative predictive value, 69.1%). Moreover, there was no significant difference in the sensitivity between the IgA nephropathy and non-IgA nephropathy groups (87.1 and 89.8% for UF-%sRBCs and 83.9 and 78.4% for Lysed-RBCs, respectively). In the NGN group, the cut-off values showed low sensitivity (56.0% for UF-%sRBCs and 44.0% for Lysed-RBCs).
Conclusions
The RBC parameters of the UF-5000, specifically UF-%sRBCs and Lysed-RBCs, showed good cut-off values for the diagnosis of GN.
Publisher
Walter de Gruyter GmbH
Subject
Biochemistry (medical),Clinical Biochemistry,General Medicine
Reference29 articles.
1. Horie, S, Ito, S, Okada, H, Kikuchi, H, Narita, I, Nishiyama, T, et al.. Japanese guidelines of the management of hematuria 2013. Clin Exp Nephrol 2014;18:679–89. https://doi.org/10.1007/s10157-014-1001-2. 2. Fitzwater, DS, Wyatt, RJ. Hematuria. Pediatr Rev 1994;15:102–8. quiz 109. https://doi.org/10.1542/pir.15-3-102. 3. Shichiri, M, Hosoda, K, Nishio, Y, Ogura, M, Suenaga, M, Saito, H, et al.. Red-cell-volume distribution curves in diagnosis of glomerular and non-glomerular haematuria. Lancet 1988;1:908–11. https://doi.org/10.1016/s0140-6736(88)91715-1. 4. Shichiri, M, Oowada, A, Nishio, Y, Tomita, K, Shiigai, T. Use of autoanalyser to examine urinary-red-cell morphology in the diagnosis of glomerular haematuria. Lancet 1986;2:781–2. https://doi.org/10.1016/s0140-6736(86)90302-8. 5. Kim, H, Kim, YO, Kim, Y, Suh, JS, Cho, EJ, Lee, HK. Small red blood cell fraction on the UF-1000i urine analyzer as a screening tool to detect dysmorphic red blood cells for diagnosing glomerulonephritis. Ann Lab Med 2019;39:271–7. https://doi.org/10.3343/alm.2019.39.3.277.
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|