Effects of storage conditions on the stability of blood-based markers for the diagnosis of Alzheimer’s disease
Author:
Mansilla Andrea1, Canyelles Marina12, Ferrer Rosa1, Arranz Javier3, Rodríguez-Baz Íñigo3, Zhu Nuole3, Rubio-Guerra Sara3ORCID, El Bounasri Shaimaa3, Sánchez Oriol3, Torres Soraya3, Fortea Juan34, Lleó Alberto34, Alcolea Daniel34, Tondo Mireia125ORCID
Affiliation:
1. Department of Biochemistry , Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute (IIB) Sant Pau , Barcelona , Spain 2. Centre of Biomedical Investigation Network for Diabetes and Metabolic Diseases (CIBERDEM) , Madrid , Spain 3. Sant Pau Memory Unit, Department of Neurology , Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute (IIB Sant Pau) Sant Pau , Barcelona , Spain 4. Centre of Biomedical Investigation Network for Neurodegenerative Diseases (CIBERNED) , Madrid , Spain 5. Comisión de Neuroquímica y Enfermedades Neurológicas, Sociedad Española de Medicina de Laboratorio , Barcelona , Spain
Abstract
Abstract
Objectives
Alzheimer’s disease (AD) is considered the most common cause of dementia in older people. Recently, blood-based markers (BBM) Aβ1-42, Aβ1-40, and phospho Tau181 (p-Tau181) have demonstrated the potential to transform the diagnosis and prognostic assessment of AD. Our aim was to investigate the effect of different storage conditions on the quantification of these BBM and to evaluate the interchangeability of plasma and serum samples.
Methods
Forty-two individuals with some degree of cognitive impairment were studied. Thirty further patients were retrospectively selected. Aβ1-42, Aβ1-40, and p-Tau181 were quantified using the LUMIPULSE-G600II automated platform. To assess interchangeability between conditions, correction factors for magnitudes that showed strong correlations were calculated, followed by classification consistency studies.
Results
Storing samples at 4 °C for 8–9 days was associated with a decrease in Aβ fractions but not when stored for 1–2 days. Using the ratio partially attenuated the pre-analytical effects. For p-Tau181, samples stored at 4 °C presented lower concentrations, whereas frozen samples presented higher ones. Concerning classification consistency in comparisons that revealed strong correlations (p-Tau181), the percentage of total agreement was greater than 90 % in a large number of the tested cut-offs values.
Conclusions
Our findings provide relevant information for the standardization of sample collection and storage in the analysis of AD BBM in an automated platform. This knowledge is crucial to ensure their introduction into clinical settings.
Funder
Instituto de Salud Carlos III Horizon 2020 Framework Programme Generalitat de Catalunya Fundació la Marató de TV3
Publisher
Walter de Gruyter GmbH
Subject
Biochemistry (medical),Clinical Biochemistry,General Medicine
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