Effects of storage conditions on the stability of blood-based markers for the diagnosis of Alzheimer’s disease

Author:

Mansilla Andrea1,Canyelles Marina12,Ferrer Rosa1,Arranz Javier3,Rodríguez-Baz Íñigo3,Zhu Nuole3,Rubio-Guerra Sara3ORCID,El Bounasri Shaimaa3,Sánchez Oriol3,Torres Soraya3,Fortea Juan34,Lleó Alberto34,Alcolea Daniel34,Tondo Mireia125ORCID

Affiliation:

1. Department of Biochemistry , Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute (IIB) Sant Pau , Barcelona , Spain

2. Centre of Biomedical Investigation Network for Diabetes and Metabolic Diseases (CIBERDEM) , Madrid , Spain

3. Sant Pau Memory Unit, Department of Neurology , Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute (IIB Sant Pau) Sant Pau , Barcelona , Spain

4. Centre of Biomedical Investigation Network for Neurodegenerative Diseases (CIBERNED) , Madrid , Spain

5. Comisión de Neuroquímica y Enfermedades Neurológicas, Sociedad Española de Medicina de Laboratorio , Barcelona , Spain

Abstract

Abstract Objectives Alzheimer’s disease (AD) is considered the most common cause of dementia in older people. Recently, blood-based markers (BBM) Aβ1-42, Aβ1-40, and phospho Tau181 (p-Tau181) have demonstrated the potential to transform the diagnosis and prognostic assessment of AD. Our aim was to investigate the effect of different storage conditions on the quantification of these BBM and to evaluate the interchangeability of plasma and serum samples. Methods Forty-two individuals with some degree of cognitive impairment were studied. Thirty further patients were retrospectively selected. Aβ1-42, Aβ1-40, and p-Tau181 were quantified using the LUMIPULSE-G600II automated platform. To assess interchangeability between conditions, correction factors for magnitudes that showed strong correlations were calculated, followed by classification consistency studies. Results Storing samples at 4 °C for 8–9 days was associated with a decrease in Aβ fractions but not when stored for 1–2 days. Using the ratio partially attenuated the pre-analytical effects. For p-Tau181, samples stored at 4 °C presented lower concentrations, whereas frozen samples presented higher ones. Concerning classification consistency in comparisons that revealed strong correlations (p-Tau181), the percentage of total agreement was greater than 90 % in a large number of the tested cut-offs values. Conclusions Our findings provide relevant information for the standardization of sample collection and storage in the analysis of AD BBM in an automated platform. This knowledge is crucial to ensure their introduction into clinical settings.

Funder

Instituto de Salud Carlos III

Horizon 2020 Framework Programme

Generalitat de Catalunya

Fundació la Marató de TV3

Publisher

Walter de Gruyter GmbH

Subject

Biochemistry (medical),Clinical Biochemistry,General Medicine

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3