Hemocytometric characteristics of COVID-19 patients with and without cytokine storm syndrome on the sysmex XN-10 hematology analyzer
Author:
Martens Remy J. H.1, van Adrichem Arjan J.1ORCID, Mattheij Nadine J. A.1, Brouwer Calvin G.2, van Twist Daan J. L.2, Broerse Jasper J. C. R.3, Magro-Checa César4, van Dongen Christel M. P.4, Mostard Rémy L. M.3, Ramiro Sofia45, Landewé Robert B. M.46, Leers Math P. G.1ORCID
Affiliation:
1. Department of Clinical Chemistry and Hematology , Zuyderland Medical Center , Heerlen & Sittard , The Netherlands 2. Department of Internal Medicine , Zuyderland Medical Center , Heerlen & Sittard , The Netherlands 3. Department of Pulmonology , Zuyderland Medical Center , Heerlen & Sittard , The Netherlands 4. Department of Rheumatology , Zuyderland Medical Center , Heerlen & Sittard , The Netherlands 5. Department of Rheumatology , Leiden University Medical Center , Leiden , The Netherlands 6. Department of Clinical Immunology and Rheumatology , Amsterdam University Medical Center , Amsterdam , The Netherlands
Abstract
Abstract
Objectives
COVID-19 is an ongoing global pandemic. There is an urgent need for identification and understanding of clinical and laboratory parameters related to progression towards a severe and fatal form of this illness, often preceded by a so-called cytokine-storm syndrome (CSS). Therefore, we explored the hemocytometric characteristics of COVID-19 patients in relation to the deteriorating clinical condition CSS, using the Sysmex XN-10 hematology analyzer.
Methods
From March 1st till May 16th, 2020, all patients admitted to our hospital with respiratory complaints and suspected for COVID-19 were included (n=1,140 of whom n=533 COVID-19 positive). The hemocytometric parameters of immunocompetent cells in peripheral blood (neutrophils [NE], lymphocytes [LY] and monocytes [MO]) obtained upon admission to the emergency department (ED) of COVID-19 positive patients were compared with those of the COVID-19 negative ones. Moreover, patients with CSS (n=169) were compared with COVID-19 positive patients without CSS, as well as with COVID-19 negative ones.
Results
In addition to a significant reduction in leukocytes, thrombocytes and absolute neutrophils, it appeared that lymphocytes-forward scatter (LY-FSC), and reactive lymphocytes (RE-LYMPHO)/leukocytes were higher in COVID-19-positive than negative patients. At the moment of presentation, COVID-19 positive patients with CSS had different neutrophils-side fluorescence (NE-SFL), neutrophils-forward scatter (NE-FSC), LY-FSC, RE-LYMPHO/lymphocytes, antibody-synthesizing (AS)-LYMPHOs, high fluorescence lymphocytes (HFLC), MO-SSC, MO-SFL, and Reactive (RE)-MONOs. Finally, absolute eosinophils, basophils, lymphocytes, monocytes and MO-FSC were lower in patients with CSS.
Conclusions
Hemocytometric parameters indicative of changes in immunocompetent peripheral blood cells and measured at admission to the ED were associated with COVID-19 with and without CSS.
Publisher
Walter de Gruyter GmbH
Subject
Biochemistry (medical),Clinical Biochemistry,General Medicine
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