Skin in the game: a review of single-cell and spatial transcriptomics in dermatological research-Reference-Cited by-同舟云学术

Skin in the game: a review of single-cell and spatial transcriptomics in dermatological research

Published:2024-04-25 Issue:10 Volume:62 Page:1880-1891
ISSN:1434-6621
Container-title:Clinical Chemistry and Laboratory Medicine (CCLM)
language:en
Short-container-title:

Author:

Schepps Samuel¹,Xu Jonathan¹,Yang Henry¹,Mandel Jenna¹,Mehta Jaanvi¹,Tolotta Julianna¹,Baker Nicole¹,Tekmen Volkan¹,Nikbakht Neda¹²,Fortina Paolo²³,Fuentes Ignacia³⁴,LaFleur Bonnie³⁵,Cho Raymond J.³⁶,South Andrew P.²³

Affiliation:

1. Department of Dermatology and Cutaneous Biology , 6559 Thomas Jefferson University , Philadelphia , PA , USA

2. Department of Pharmacology, Physiology and Cancer Biology , 6559 Thomas Jefferson University , Philadelphia , PA , USA

3. International Federation of Clinical Chemistry Working Group on Single Cell and Spatial Transcriptomics , Milan , Italy

4. Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas , Pontificia Universidad Católica de Chile, Directora de Investigación Fundación DEBRA Chile , Santiago , Chile

5. R. Ken Coit College of Pharmacy , University of Arizona, University of Arizona Cancer Center , Tucson , AZ , USA

6. Department of Dermatology , University of San Francisco , San Francisco , CA , USA

Abstract

Abstract Single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) are two emerging research technologies that uniquely characterize gene expression microenvironments on a cellular or subcellular level. The skin, a clinically accessible tissue composed of diverse, essential cell populations, serves as an ideal target for these high-resolution investigative approaches. Using these tools, researchers are assembling a compendium of data and discoveries in healthy skin as well as a range of dermatologic pathophysiologies, including atopic dermatitis, psoriasis, and cutaneous malignancies. The ongoing advancement of single-cell approaches, coupled with anticipated decreases in cost with increased adoption, will reshape dermatologic research, profoundly influencing disease characterization, prognosis, and ultimately clinical practice.

Publisher

Walter de Gruyter GmbH

Link

https://www.degruyter.com/document/doi/10.1515/cclm-2023-1245/pdf

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