Zum Mechanismus der Hydrolyse des Adenosin-3′.5′-cyclophosphatbenzylesters / On the Mechanism of Adenosine-3′,5′-cyclophosphatebenzylester Hydrolysis

Abstract

The hydrolysis of benzyltriesters of adenosine 3′,5′-cyclophosphate with H2 18O was undertaken. The mass spectra of the benzylalcohol fragments contained the label whereas the cAMP after derivatisation with trimethylsilyl-groups did not show an enhanced M + 2 peak. This clearly indicates that the benzylic C–O bond is broken during hydrolysis.