Affiliation:
1. Department of Clinical Laboratory Science , The 117th Hospital of PLA , Hangzhou 310013 , China
Abstract
Abstract
Background
This research is aimed to study the resistance and molecular epidemiological characteristics of carbapenem-resistant Klebsiella pneumoniae (CRKP).
Methodology
38 isolated CRKP strains were collected from clinical specimens.
Results
The resistance rates were more than 70.0%. Ampicillin had the highest rates among them (100.0%). 34 strains (89.5%) among the 38 CRKP strains carried bla
KPC-2 gene, and 3 strains (7.9%) carried bla
IMP-4 gene. 36 strains (94.7%) among the 38 CRKP strains carried bla
SHV gene, 29 strains (76.3%) carried bla
TEM gene, and 26 strains (68.4%) carried bla
CT-M gene. 7 strains (18.4%) among the 38 CRKP strains carried bla
DHA-1 gene. 15 strains (39.5%) in 38 CRKP strains lost two fenestra proteins, ompK35 and ompK36, and the rest 23 strains carried ompK36 genes. 38 CRKP strains were divided into five kinds of ST types, with ST11 type as the most (86.8%, 33/38). The rest of the ST types included 2 strains of ST23 (5.3%, 2/38), one strain of ST15, ST1373 and ST1415 (2.6%, 1/38).
Conclusions
CRKP resistance is severe, and the mechanism of drug resistance has become increasingly complex. Various ST types and resistance genes are related to CRKP. The clinical prevention and control work is imminent.
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