New generation sequencing: molecular approaches for the detection and monitoring of bioaerosols in an indoor environment: a systematic review
Author:
Onwusereaka Cynthia Oluchi1, Jalaludin Juliana1, Oluchi Sampson Emilia2, Poh Choo Veronica Chua3
Affiliation:
1. Department of Environmental and Occupational Health, Faculty of Medicine and Health Sciences , Universiti Putra Malaysia Serdang , Selangor , Malaysia 2. Department of Community Health, Faculty of Medicine and Health Science , Universiti Putra Malaysia Serdang , Selangor , Malaysia 3. Faculty of Health Sciences , MAHSA University Jenjarom , Selangor , Malaysia
Abstract
Abstract
Introduction
The exposure of occupants to indoor air pollutants has increased in recent decades. The aim of this review is to discuss an overview of new approaches that are used to study fungal aerosols. Thus, this motivation was to compensate the gaps caused by the use of only traditional approaches in the study of fungal exposure.
Content
The search involved various databases such as; Science Direct, PubMed, SAGE, Springer Link, EBCOHOST, MEDLINE, CINAHL, Cochrane library, Web of Science and Wiley Online Library. It was limited to full text research articles that reported the use of non-viable method in assessing bioaerosol, written in English Language, full text publications and published from year 2015–2022.
Summary and Outlook
A total of 15 articles met the inclusion criteria and was included in this review. The use of next-generation sequencing, which is more commonly referred to as high-throughput sequencing (HTS) or molecular methods in microbial studies is based on the detection of genetic material of organisms present in a given sample. Applying these methods to different environments permitted the identification of the microorganisms present, and a better comprehension of the environmental impacts and ecological roles of microbial communities. Based on the reviewed articles, there is evidence that dust samples harbour a high diversity of human-associated bacteria and fungi. Molecular methods such as next generation sequencing are reliable tools for identifying and tracking the bacterial and fungal diversity in dust samples using 18S metagenomics approach.
Funder
Ministry of Higher Education Malaysia under the Fundamental Research Grant Scheme
Publisher
Walter de Gruyter GmbH
Subject
Public Health, Environmental and Occupational Health,Pollution,Health (social science)
Reference109 articles.
1. Canha, N, Almeida, SM, Freitas, MD, Wolterbeek, HT. Assessment of bioaerosols in urban and rural primary schools using passive and active sampling methodologies. Arch Environ Protect 2015;41. https://doi.org/10.1515/aep-2015-0034. 2. Deng, W, Chai, Y, Lin, H, So, WW, Ho, KW, Tsui, AK, et al.. Distribution of bacteria in inhalable particles and its implications for health risks in kindergarten children in Hong Kong. Atmos Environ 2016;128:268–75. https://doi.org/10.1016/j.atmosenv.2016.01.017. 3. Leppänen, HK, Täubel, M, Roponen, M, Vepsäläinen, A, Rantakokko, P, Pekkanen, J, et al.. Determinants, reproducibility, and seasonal variation of bacterial cell wall components and viable counts in house dust. Indoor Air 2015;25:260–72. https://doi.org/10.1111/ina.12141. 4. Horner, WE. Assessment of the indoor environment: evaluation of mold growth indoors. Immunol Allergy Clin 2003;23:519–31. https://doi.org/10.1016/s0889-8561(03)00063-8. 5. Mendell, MJ, Mirer, AG, Cheung, K, Tong, M, Douwes, J. Respiratory and allergic health effects of dampness, mold, and dampness-related agents: a review of the epidemiologic evidence. Environ Health Perspect 2011;119:748–56. https://doi.org/10.1289/ehp.1002410.
|
|