Affiliation:
1. Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University , P.O. Box 173 , Al-Kharj 11942 , Saudi Arabia
2. Department of Pharmaceutics, College of Pharmacy, King Saud University , P.O. Box 2457 , Riyadh 11451 , Saudi Arabia
3. Department of Pharmaceutical Sciences, College of Pharmacy, AlMaarefa University , P.O. Box 71666, Diriyah 13713 , Riyadh , Saudi Arabia
Abstract
Abstract
A fast, sensitive, and green reverse-phase “high-performance thin-layer chromatography” approach for the simultaneous estimation of ibuprofen (IBF), caffeine (CAF), and paracetamol (PCM) in marketed formulations was established and verified in this study. The binary combination of acetone and water (80:20 v/v) was used as the green eluent system. The current method’s greenness was predicted using four different approaches, namely National Environmental Method Index, Analytical Eco-Score (89), ChlorTox (1.08 g), and the Analytical GREENness (83) approaches, which demonstrated an outstanding greener profile. The present approach was linear in the range of 25–800 ng·band−1 for the simultaneous estimation of IBF, CAF, and PCM. In addition, the current method was accurate (% recoveries = 100 ± 2), precise (%CV < 2%), robust (%CV < 2), sensitive (LOD = 1.13–2.71 ng·band−1 and LOQ = 3.39–8.10 ng·band−1), and green. The amount of IBF, CAF, and PCM in commercial tablets was determined to be 99.51%, 98.25%, and 100.64%, respectively. The present method for the simultaneous determination of IBF, CAF, and PCM in marketed tablets is supported by these data. The findings of this study suggested that the current approach may be consistently applied to analyze IBF, CAF, and PCM in marketed tablets.