Biofabrication of silver nanoparticles using Uncaria tomentosa L.: Insight into characterization, antibacterial activities combined with antibiotics, and effect on Triticum aestivum germination

Author:

Alghanmi Reem M.1,Hamouda Ragaa A.23,Al-Moubaraki Aisha H.1,Allouzi Afnan A.1,Abuelmagd Muhammad A.45

Affiliation:

1. Department of Chemistry, College of Science, University of Jeddah , Jeddah 21589 , Saudi Arabia

2. Department of Biology, College of Sciences and Arts Khulais, University of Jeddah , Jeddah , Saudi Arabia

3. Genetic Engineering and Biotechnology Research Institute, University of Sadat City , Sadat City , Egypt

4. Department of Botany, Faculty of Science, Mansoura University , Mansoura , Egypt

5. Faculty of Pharmacy, Mansoura National, Egypt University , Gamasa 7731168 , Egypt

Abstract

Abstract Herein, we used the aqueous extract of Uncaria tomentosa L. barks (Cat’s claw bark [CCb]) for the biofabrication of silver nanoparticles (CCb-Ag-NPs). The effects of different parameters (Uncaria tomentosa L. aqueous extract, silver nitrate [AgNO3] ratio, temperature, and pH) on the formation of the nanoparticles were investigated using UV scan as a preliminary tool for the detection of surface plasmon resonance of CCb-Ag-NPs. The optimal ratio was 1:7 (Uncaria tomentosa L. extract: 1 mM AgNO3 solution). Fourier-transform infrared spectroscopy revealed the functional groups of both CCb extract and the CCb-Ag-NPs, whose dispersion and quasispherical morphologies were characterized using scanning electron microscopy and transmission electron microscopy. Particle sizes ranged from 19.2 to 38.5 nm. The zeta potential of CCb-Ag-NPs was −34.44 mV. According to energy-dispersive X-ray analysis, the CCb-Ag-NPs contained 28.87% silver. The formation of Ag-NPs was also confirmed by X-ray diffraction pattern analysis. Pristine CCb-Ag-NPs showed antibacterial activity against three pathogenic bacterial strains: Escherichia coli (ATCC 25922), E. coli (ATCC 8739), and Pseudomonas aeruginosa (ATCC 90274). Antibacterial activity increased significantly after loading CCb-Ag-NPs on antibiotic discs containing meropenem and cefoxitin. Low concentrations of CCb-Ag-NPs also enhanced the germination percentage, coleoptile length, and radical root length of Triticum aestivum.

Publisher

Walter de Gruyter GmbH

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