Cryo secondary ion mass spectrometry for wood component visualization: a mini review
Author:
Aoki Dan1ORCID, Matsushita Yasuyuki2ORCID, Fukushima Kazuhiko1
Affiliation:
1. Graduate School of Bioagricultural Sciences, Nagoya University , Nagoya 464-8601 , Japan 2. Institute of Agriculture, Tokyo University of Agriculture and Technology , Tokyo 183-8509 , Japan
Abstract
Abstract
Various phenomena in living physiological systems are conducted on the hydrated conditions, and in many cases, they do not work in a dry state. Imaging mass spectrometry is one of the direct detection methods scanning the sample surface with some focused and pulsed energy and analysing the sputtered components. However, under the high vacuum conditions required for usual imaging mass spectrometry, the sample surface is rapidly dried. It is difficult for the target cell to survive, and the original situation are lost soon. Here, the combination of a freeze-fixation and a cryo sample stage is a promising method to do mass spectrometry while maintaining the original situation. By rapidly freezing the cells, the momentary situation as a living cell is fixed. The situation in a living cell can be captured as still images by cryo imaging mass spectrometry. This mini-review introduces the outline of imaging mass spectrometry especially for low molecular weight components and recent results for frozen-hydrated samples by cryo secondary ion mass spectrometry.
Publisher
Walter de Gruyter GmbH
Reference71 articles.
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