Reinigung und Charakterisierung zweier Proteine des Adenovirus Typ 2

Abstract

Two soluble antigens produced in KB-cells after infection with Adenovirus type 2 were identified with constituents of the virion itself. Purification and concentration of the two proteins (named A- and C-Protein according to PEREIRA) is described. The sedimentation rate of the A-Protein is 11.5 S, which gives a molecular weight of 310 000, and the corresponding data for the C-Protein are 5.5 S and 81 000 molecular weight. The A-Proteins of different types, 2, 3 and 5 are physically similar, but serologically composed of parts with similar and parts with type-specific differences.