Selektive Anreicherung von Staphylokokken-Panton-Valentine-Leukozidin mit Aluminiumoxid-Cholesterin / Selective Enrichment of Staphylococcus “Panton-Valentine”-Leukocidine with Aluminium Oxide Cholesterol

Author:

Kanoe M.1,Blobel H.1,Schaeg W.1

Affiliation:

1. Institut für Bakteriologie und Immunologie der Veterinärmedizinischen Fakultät der Justus Liebig-Universität Gießen (Deutschland)

Abstract

A selective concentration of “Panton-Valentine”-leukozidine (PVL) from the culture-supernatant Staphylococcus aureus was achieved through adsorption of most of the a-hemolysin, coagulase, eggyolk-opacity factor and fibrinolysin to aluminiumoxide cholesterol. PVL was further concentrated through dialysis against a saturated ammonium sulfate solution and subsequent pressure-filtration. Through pressure-filtration relatively low-molecular staphylococcal substances, such as a part of a nuclease, could be separated from PVL.

Publisher

Walter de Gruyter GmbH

Subject

General Chemistry

Cited by 3 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Purification of oligomeric staphylococcal α-toxin by affinity chromatography on Digitonin-Sepharose;Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A: Medical Microbiology, Infectious Diseases, Virology, Parasitology;1985-02

2. Staphylococcal Leukocidin, Panton-Valentine Type;Journal of Toxicology: Toxin Reviews;1985-01

3. Effects of Panton-Valentine Leukocidin of Staphylococcus aureus on Leukocytes from Patients with Leukemia;Infection and Immunity;1971-04

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