The Phosphatidylinositol Species of Suspension Cultured Plant Cells

Abstract

Suspension cultured Nicotiana tabacum and Catharanthus roseus cells were labeled with [3H]inositol, the phospholipid fraction extracted and separated by thin layer chromatography. Three different solvent systems and reference compounds were used to assign the different 3H-labeled species by autoradiography. The ratio of [3H]inositol incorporation into PI, PIP and PIP2 was found to be 95:4:1; with some preparations a lyso-PI band was obtained which incorporated about a tenth of the label of the PIP band. With Catharanthus roseus cells a very faint band between PI and lyso-PI was detected which could not be assigned to a reference compound.