Author:
Nexo Ebba,Nooroya Ben,Hvas Anne-Mette,Christensen Anna Lisa,Waters Harry
Abstract
AbstractThe aim was to develop and validate an assay for measuring autoantibodies against human intrinsic factor (IF). For this purpose 1.25pmol of recombinant IF was coupled to each well. Samples (100μL of plasma diluted 1:1) were incubated for 1h, followed by 30min with the detection reagent (biotinylated IF, 3.3pmol/mL). Samples were obtained from healthy blood donors (n=141) and patients with suspected vitamin B
Subject
Biochemistry, medical,Clinical Biochemistry,General Medicine
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