Affiliation:
1. Henan International Joint Laboratory of Medicinal Plants Utilization, College of Chemistry and Chemical Engineering , Henan University , Kaifeng , China
2. Key Laboratory of Natural Medicine and Immune-Engineering of Henan Province , Henan University , Kaifeng , China
3. School of Life Science , Henan University , Kaifeng , China
Abstract
Abstract
Background
Lonicera japonica Thunb has been comprehensively used as a traditional Chinese medicine which contains a variety of medicinally active substances.
Objectives
The active constituents in various parts of L. japonica Thunb (leaves [LE], flowers fl, and green buds [GB]) were studied to rational and efficient use of this medicinal plant.
Methods
UV–vis spectrophotometry was used for the determination of the total flavonoids; gas chromatography–mass spectrometric method was employed for the analysis of volatile components; and high-performance liquid chromatography technology was applied to detect nine bioactive components including chlorogenic acid, neochlorogenic acid, isochlorogenic acid C, caffeic acid, luteolin, galuteolin, hyperoside, rutin, and quercetin in different parts of L. japonica Thunb. The antimicrobial effects of LE, FL, and GB were further measured using agar plate drilling method.
Results
The content of the total flavonoids in different portions of L. japonica Thunb has significant difference, and the content with the decreasing sequence is found in GB, LE, and FL. The contents of main volatile components were highest in FL, followed by GB and LE. Besides, the contents of galuteolin, luteolin, rutin, and isochlorogenic acid C were higher in LE than those in FL and GB, and neochlorogenic acid content was similar in LE and FL. The antimicrobial effects showed that LE had the best inhibitory activity on Staphylococcus aureus (SA) and extended spectrum β-lactamases SA.
Conclusions
The research in this study provided valuable evidences for the reasonable and efficient utilization of L. japonica Thunb.
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