Abstract
Abstract
Objective
The effects of urapidil in intestinal ischemia-reperfusion (IR) model were investigated using histopathological and biochemical methods.
Materials and methods
Forty Wistar albino rats were subjected to sham operation (Group 1), IR (Group 2), IR+dimethyl sulfoxide (Group 3), IR+urapidil 0.5 mg/kg (Group 4), and IR+urapidil 5 mg/kg (Group 5). Levels of MDA, TAS, TOS, SOD, MPO, NF-κB, caspase-3, and LC3B were measured.
Results and discussion
The groups 2 and 3 had significantly higher TOS and MPO levels than the sham group had (p < 0.001), whereas the TAS and SOD levels were significantly lower in Group 2 than in the sham group. In treatment groups, TAS and SOD levels increased, whereas TOS, MPO, and MDA levels decreased compared to Group 2. Caspase-3 and LC3B immunopositivities were seen at severe levels in Group 2 and 3. However, Group 4 and 5 were found to have lower levels of immunopositivity. Immunopositivity was observed in interstitial areas, peribronchial region, and bronchial epithelial cells. A moderate level of NF-κB immunopositivity was seen in Group 2 and 3.
Conclusion
Our results show that urapidil is one of the antioxidant agents and protects lung tissue from oxidant effects of intestinal IR injury.
Subject
Biochemistry (medical),Clinical Biochemistry,Molecular Biology,Biochemistry
Reference94 articles.
1. Evidence for neutrophil-related acute lung injury after intestinal ischemia-reperfusion;Surgery,1989
2. Acute intestinal ischemia and infarction;Seminars in gastrointestinal disease,2003
3. Ginsenoside Rg1 protects mouse liver against ischemia–reperfusion injury through anti-inflammatory and anti-apoptosis properties;J Surg Res,2014
4. Emerging roles of caspase-3 in apoptosis;Cell Death Differ,1999
5. Urapidil;Drugs,1989
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