The protective effects of urapidil on lung tissue after intestinal ischemia-reperfusion injury

Author:

Ozturk Deniz,Guzel Erdogan DeryaORCID,Tanyeli Ayhan,Çomaklı Selim,Baylan Hüseyin,Polat Elif

Abstract

Abstract Objective The effects of urapidil in intestinal ischemia-reperfusion (IR) model were investigated using histopathological and biochemical methods. Materials and methods Forty Wistar albino rats were subjected to sham operation (Group 1), IR (Group 2), IR+dimethyl sulfoxide (Group 3), IR+urapidil 0.5 mg/kg (Group 4), and IR+urapidil 5 mg/kg (Group 5). Levels of MDA, TAS, TOS, SOD, MPO, NF-κB, caspase-3, and LC3B were measured. Results and discussion The groups 2 and 3 had significantly higher TOS and MPO levels than the sham group had (p < 0.001), whereas the TAS and SOD levels were significantly lower in Group 2 than in the sham group. In treatment groups, TAS and SOD levels increased, whereas TOS, MPO, and MDA levels decreased compared to Group 2. Caspase-3 and LC3B immunopositivities were seen at severe levels in Group 2 and 3. However, Group 4 and 5 were found to have lower levels of immunopositivity. Immunopositivity was observed in interstitial areas, peribronchial region, and bronchial epithelial cells. A moderate level of NF-κB immunopositivity was seen in Group 2 and 3. Conclusion Our results show that urapidil is one of the antioxidant agents and protects lung tissue from oxidant effects of intestinal IR injury.

Publisher

Walter de Gruyter GmbH

Subject

Biochemistry (medical),Clinical Biochemistry,Molecular Biology,Biochemistry

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