Enteroprotective effect of Tsukamurella inchonensis on streptozotocin induced type 1 diabetic rats

Abstract

Abstract Objectives Enteropathy is one of the most important complications of diabetes mellitus. The present study determined the possible effects of Tsukamurella inchonensis (Ti) on diabetes enteropathy on rat small intestine. Materials and methods A total of 40 rats were divided into four groups of 10. Diabetes was induced by streptozotocin. Oral administration of Ti at dose of 105 and 107 CFU/rat was performed in two groups continuously for 14 days. The third and fourth groups received normal saline as the diabetic and negative control groups, respectively. The blood and intestine tissue samples were taken on 21st day post treatment for biochemical and pathological evaluations. Results Significant differences were found in serum glucose, cholesterol and triglycerides values together with in CAT and SOD activities, MDA level and IL-6 concentration in both Ti treated groups in comparison with the diabetic rats. Moreover, there were severe pathological changes including degeneration of intestinal mucosa, mononuclear cell infiltration, decreasing number of goblet cells and villous length associated with increasing in villous thickness on the diabetic rats which markedly attenuated in both Ti recipient groups. Conclusion In conclusion, it seems that oral administration of Ti may improve intestinal damage in diabetic patients by modulation of intestinal antioxidant defense system.