Characterization of glial-restricted precursors from rhesus monkey embryonic stem cells-Reference-Cited by-同舟云学术

Characterization of glial-restricted precursors from rhesus monkey embryonic stem cells

Published:2015-01-01 Issue:1 Volume:6 Page:244-251
ISSN:2081-6936
Container-title:Translational Neuroscience
language:
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Author:

Chen Hongwei¹²³⁴,Mao Yu⁵⁴,Wang Shufen¹²⁴,Li Bin¹²⁴,Wang Jinhuan⁶,Li Jian⁷,Ma Yuanye⁵

Affiliation:

1. 1Laboratory of Reproductive and Developmental Biology, Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R.China

2. 3Yunnan Key Laboratory of Animal Reproductive Biology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R.China

3. 4Stem Cell and Brain Research Institute, INSERM U846, Bron 69675, France

4. 7Graduate University of Chinese Academy of Sciences, Beijing100049, P. R.China

5. 2Laboratory of Primate Recognition Neurosciences, Kunming Primate Research Center, and Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R.China

6. 5Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R.China

7. 6Central Laboratory, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P. R.China

Abstract

AbstractGlial-restricted precursor (GRP) cells, the earliest glial progenitors for both astrocytes and oligodendrocytes, have been derived from embryos and embryonic stem cells (ESC) in rodents. However, knowledge regarding the equivalent cell type in primates is limited due to restrictions imposed by ethics and resources. Here we report successful derivation and characterization of primate GRP cells from rhesus monkey ESC. The purified monkey GRP cells were A2B5-positive and FGF2-dependent for survival and proliferation. The differentiation assays indicated that they were tri-potential in vitro and bi-potential in vivo. These newly purified GRP cells will help to facilitate understanding of the molecular mechanism of glial development in primates as well as provide a source of therapeutic donor cells for use in neuroregenerative medicine.

Publisher

Walter de Gruyter GmbH

Subject

General Neuroscience

Link

https://www.degruyter.com/document/doi/10.1515/tnsci-2015-0026/pdf

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