Trehalose improves the movement ability of Aβ<sub>arc</sub> <i>Drosophila</i> by restoring the damaged mitochondria-Reference-Cited by-同舟云学术

Trehalose improves the movement ability of Aβ_arc Drosophila by restoring the damaged mitochondria

Published:2024-01-01 Issue:1 Volume:15 Page:
ISSN:2081-6936
Container-title:Translational Neuroscience
language:en
Short-container-title:

Author:

Li Liangxian¹²,Huang Zhiheng¹³,Wu Mingli²,Li Xia²,Xiao Bo³⁴,Yao Dong¹⁵⁶,Mo Biwen¹⁵⁶

Affiliation:

1. Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Guilin Medical University , Guilin , 541199 , China

2. Guangxi Key Laboratory of Brain and Cognitive Neuroscience, Guilin Medical University , Guilin , 541199 , China

3. Laboratory of Respiratory Disease, Affiliated Hospital of Guilin Medical University , Guilin , 541002 , China

4. Laboratory of Basic Research on Respiratory Diseases, Guangxi Health Commission, Guilin Medical University , Guilin , 541199 , China

5. Guangxi Clinical Research Center for Diabetes and Metabolic Diseases, Guangxi Health Commission Key Laboratory of Glucose and Lipid Metabolism Disorders, The Second Affiliated Hospital of Guilin Medical University , 541199 , Guilin , China

6. Guangxi Key Laboratory of Metabolic Reprogramming and Intelligent Medical Engineering for Chronic Diseases, The Key Laboratory of Respiratory Diseases, Education Department of Guangxi Zhuang Autonomous Region, Guilin Medical University , 541199 , Guilin , China

Abstract

Abstract Background The deposition of Aβ42 has been regarded as one of the important pathological features of Alzheimer’s disease (AD). However, drug development for Aβ42 toxicity has been progressed slowly. Objective Our aim was to introduce the effect and related mechanism of trehalose on an Aβarc (arctic mutant Aβ42) Drosophila AD model. Methods The human Aβarc was expressed in Drosophila to construct the AD model. Trehalose was added to the culture vial. The movement ability was determined by detecting climbing ability and flight ability. Enzyme-linked immunosorbent assay was used to detect the levels of Aβarc, ATP, and lactate. Electron microscopy assay, mitochondrial membrane potential assay, and mitochondrial respiration assay were used to assess the mitochondrial structure and function. Results Trehalose strongly improved the movement ability of Aβarc Drosophila in a concentration gradient-dependent manner. Furthermore, trehalose increased the content of ATP and decreased the content of Aβarc and lactate both in the brain and thorax of Aβarc Drosophila. More importantly, the mitochondrial structure and function were greatly improved by trehalose treatment in Aβarc Drosophila. Conclusion Trehalose improves movement ability at least partly by reducing the Aβarc level and restoring the mitochondrial structure and function in Aβarc Drosophila.

Publisher

Walter de Gruyter GmbH

Link

https://www.degruyter.com/document/doi/10.1515/tnsci-2022-0338/pdf

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