Affiliation:
1. Department of Neurology, The Affiliated Shanghai Songjiang Central Hospital of Shanghai Jiao Tong University, Central Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai, China
Abstract
AbstractAimThe aim of this study was to investigate the neuroprotective effects of
hydrochloride fasudil (HF) in rats following intracerebral hemorrhage (ICH).MethodsMale Wistar rats were randomly divided into four groups: normal, sham-operated,
ICH, and ICH/HF. ICH was induced by injection of non-anticoagulant autologous
arterial blood into the right caudate nucleus. The levels of Rho-associated
protein kinase 2 (ROCK2) mRNA and protein around the site of the hematoma were
measured by quantitative real-time polymerase chain reaction and enzyme-linked
immunosorbent assay (ELISA), respectively. The levels of interleukin-6 and tumor
necrosis factor-α in serum were detected by ELISA. The inflammatory cells
and changes in the neuronal morphology around the hematoma were visualized using
hematoxylin and eosin and Nissl staining. Brain edema was measured by comparing
wet and dry brain weights.ResultsFollowing ICH, the levels of ROCK2 were significantly increased from day 1 to day
7. The levels of ROCK2 were significantly lower in rats treated with HF than in
controls. The levels of inflammatory cytokines and brain water content were
significantly higher in rats treated with HF than in controls. Administration of
HF significantly reduced the levels of inflammatory cytokines and brain water
content from day 1 to day 7. In the acute phase of ICH, a large number of
neutrophils infiltrated the perihematomal areas. In comparison with the ICH group,
the ICH/HF group showed markedly fewer infiltrating neutrophils on day 1. Nissl
staining showed that ICH caused neuronal death and loss of neurons in the
perihematomal areas at all time points and that treatment with HF significantly
attenuated neuronal loss.ConclusionsHF exerts neuroprotective effects in ICH rats by inhibiting the expression of
ROCK2, reducing neutrophil infiltration and production of inflammatory cytokines,
decreasing brain edema, and attenuating loss of neurons.
Cited by
10 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献