Inhibition of Succinic Dehydrogenase and F0F1-ATP Synthase by 4,4'-Diisothiocyanatostilbene-2,2'-disulfonic Acid (DIDS)

Author:

Bernardes Celene F.1,Meyer-Fernandes Jose R.2,Martins Orlando B.3,Vercesi Anibal E.4

Affiliation:

1. Departamento de Patologia Ch'nica, Faculdade de Ciencias Medicas, Universidade Estadual de Campinas, 13084-100, CP 6111, Campinas, SP. Brazil

2. Departamento de Bioquímica Medica, Universidade Federal do Rio de Janeiro, 21941-590, Rio de Janeiro, RJ, Brazil

3. Departamento de Bioquímica Médica, Universidade Federal do Rio de Janeiro, 21941-590, Rio de Janeiro, RJ, Brazil

4. Departamento de Patologia Clínica, Faculdade de Ciencias Médicas, Universidade Estadual de Campinas, 13084-100, CP 6111, Campinas, SP. Brazil

Abstract

Abstract This study shows that incubation of rat liver mitochondria in the presence of the thiol/ amino reagent 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DID S) is followed by inhibition of both succinate supported respiration and oxidative phosphorylation. Half-maximal inhibition of succinic dehydrogenase activity and succinate oxidation by mitochondria was attained at 55.3 and 60.8 μm DIDS, respectively. DIDS did inhibit the net ATP synthesis and ATP ⇔ [32P]Pi exchange reaction catalyzed by submitochondrial particles in a dose-dependent manner (Ki= 31.7 μm and Ki = 32.7 μm), respectively. The hydrolytic activities of uncoupled heart submitochondrial particles and purified F 1 -ATPase were also inhibited 50% by 31.9 and 20.9 μm DIDS, respectively.

Publisher

Walter de Gruyter GmbH

Subject

General Biochemistry, Genetics and Molecular Biology

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