Comparison of two in vitro methods for the detection of ivermectin resistance in Haemonchus contortus in sheep-Reference-Cited by-同舟云学术

Comparison of two in vitro methods for the detection of ivermectin resistance in Haemonchus contortus in sheep

Published:2016-04-22 Issue:2 Volume:53 Page:120-125
ISSN:1336-9083
Container-title:Helminthologia
language:en
Short-container-title:

Author:

Urda Dolinská M.¹,Königová A.¹,Babják M.¹,Várady M.¹

Affiliation:

1. Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01 Košice, Slovak Republic

Abstract

Summary Gastrointestinal parasitic nematodes in sheep cause severe economic losses. Anthelmintics are the most commonly used drugs for prophylaxis and therapy against parasitic helminths. The problem of drug resistance has developed for all commercially available anthelmintics in several genera and classes of helminths. In vitro and in vivo tests are used to detect anthelmintic resistance. Two in vitro methods (larval migration inhibition test and micromotility test) for the detection of ivermectin (IVM) resistance were compared using IVM-resistant and IVM-susceptible isolates of Haemonchus contortus. The degree of resistance for each test was expressed as a resistance factor (RF). The micromotility test was more sensitive for quantitatively measuring the degree of resistance between susceptible and resistant isolates. The RFs for this test for IVM and eprinomectin ranged from 1.00 to 108.05 and from 3.87 to 32.32, respectively.

Publisher

Walter de Gruyter GmbH

Subject

Animal Science and Zoology,Parasitology

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