Morphological and molecular characterization of Xiphinema species from Shenzhen, China
Author:
Zeng Y., Ye W.1, Zhang Z.2, Sun H.2, Yong L.3, Huang Y.4, Zhao K.3, Liang H.3, Kerns J.2
Affiliation:
1. Nematode Assay Section, Agronomic Division, North Carolina Department of Agriculture and Consumer Services, Raleigh, NC 27607, USA 2. Department of Plant Protection, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, People’s Republic of China 3. Shenzhen Environmental Monitoring Center, Shenzhen 518049, People’s Republic of China 4. College of Life Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, People’s Republic of China
Abstract
Summary
During a nematode biodiversity survey from 2012 to 2014 in Shenzhen, China, ten nematode populations (SZX1301–SZX1310) of Xiphinema were recovered from rhizosphere of different plants, namely Acacia mangium (SZX1306), A. confuse (SZX1309), Blechnum orientale (SZX1301, SZX1302, SZX1307, SZX1308), Litchi chinensis (SZX1304, SZX1310) in Tianxinshan and Gleichenia linearis (SZX1303, SZX1305) in Yangmeikeng environmental monitoring sites. Morphological and molecular profiles of these populations were determined. Three species of Xiphinema, i.e., X. hunanienseWang & Wu, 1992, X. brasilienseLordello, 1951 and X. americanum Cobb, 1913 sensu lato were identified using morphological characters and molecular data of partial 18S and 28S D2–D3 rDNA expansion segments. Four populations (SZX1301–SZX1304) were X. hunaniense, one population (SZX1305) X. brasiliense, and five populations (SZX1306–SZX1310) X. americanum s.l.. Phylogenetic analysis based on sequences of the 28S rDNA D2–D3 expansion segment revealed these three species are all distinct species and supported a close relationship with their corresponding species. This is the first report of X. hunaniense, X. brasiliense and X. americanum s.l. in their hosts except for L. chinensis.
Publisher
Walter de Gruyter GmbH
Subject
Animal Science and Zoology,Parasitology
Reference73 articles.
1. Alkemade, J. R. M., Loof, P. A. A. (1990): The genus Xiphinema Cobb, 1913 (Nematoda: Longidoridae) in Peru. Rev. Nématol., 13: 339 – 348 2. Barsi, L., DE Luca, F. (2008): Morphological and molecular characterization of two putative Xiphinema americanum-group species, X. parasimile and X. simile (Nematoda: Dorylaimida) from Serbia. Nematology, 10: 15 – 25 3. Brodie, B. B., Good, J. M., Adams, W. E. (1969): Population dynamics of plant nematodes in cultivated soil: effect of sod-based rotations in cecil sandy loam. J. Nematol., 1: 309 – 312 4. Brown, D. J. F., Boag, B. (1988): An examination of methods used to extract virus-vector nematodes (Nematoda: Longidoridae and Trichodoridae) from soil samples. Nematol. Mediterr., 16: 93 – 99 5. Brown, D. J. F., Topham, P. B. (1985): Morphometric variability between populations of Xiphinema diversicaudatum (Nematoda: Dorylaimoidea). Rev. Nematol., 8: 15 – 26
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