Quantitative assessment of xylan distribution across the secondary cell wall layers of Eucalyptus dissolving pulp fibres
Author:
Lekha Prabashni1, Bush Tamara2, Pammenter Norman3, Sitholè Bruce45, Berjak Patricia3
Affiliation:
1. Council for Scientific and Industrial Research (CSIR), Biorefinery Industry Development Facility , 359 Masizi Kunene Avenue , Durban 4013 , South Africa , Phone: +2731 242 2331/+2731 261 1216 2. School of Chemistry , University of KwaZulu-Natal (UKZN) , Durban 4001 , South Africa 3. School of Life Sciences , UKZN , Durban 4001 , South Africa 4. Council for Scientific and Industrial Research (CSIR), Biorefinery Industry Development Facility , 359 Masizi Kunene Avenue , Durban 4013 , South Africa 5. Discipline of Chemical Engineering , UKZN , Durban 4001 , South Africa
Abstract
Abstract
A quantitative method has been developed for assessment of the distribution of xylan across the secondary cell wall layers of Eucalyptus dissolving pulp fibres by means of a carbohydrate binding module (CBM), CtCBM6, in combination with transmission electron microscopy (TEM). To ensure reproducibility and to minimise non-specific labelling, various parameters were optimised, namely the size of the gold colloid marker, CtCBM6 concentration, and the selection of buffer solutions. The method was replicated on processed Eucalyptus fibres containing different xylan contents. Reproducible xylan counts and distributions across the secondary cell wall layers were obtained for unbleached and bleached Eucalyptus fibres. The xylan distribution pattern across the cell wall layers S1, S2 and S3 was similar, but the S1 and S3 layers contained after bleaching more xylan than the S2 layer. The technique has a wide range of applications in basic wood research as well as in the analysis of technological processes.
Publisher
Walter de Gruyter GmbH
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