The phosphoinositide PI(3,5)P<sub>2</sub> inhibits the activity of plant NHX proton/potassium antiporters: Advantages of a novel electrophysiological approach-Reference-Cited by-同舟云学术

The phosphoinositide PI(3,5)P₂ inhibits the activity of plant NHX proton/potassium antiporters: Advantages of a novel electrophysiological approach

Published:2022-01-01 Issue:1 Volume:13 Page:119-125
ISSN:1868-503X
Container-title:Biomolecular Concepts
language:en
Short-container-title:

Author:

Gradogna Antonella¹,Pardo José M.²,Carpaneto Armando³¹

Affiliation:

1. Institute of Biophysics, National Research Council , Via De Marini 6 , 16149 Genova , Italy

2. Institute of Plant Biochemistry and Photosynthesis, Consejo Superior de Investigaciones Cientificas and University of Seville , Seville 41092 , Spain

3. Department of Earth, Environment and Life Sciences (DISTAV) – University of Genoa , Viale Benedetto XV 5 , 16132 Genova , Italy

Abstract

Abstract In the present work, we discuss the way in which the parallel application of the patch-clamp technique and the 2′,7′-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) fluorescence detection for recording luminal proton changes allows the functional characterization of nonelectrogenic potassium/proton vacuolar antiporters of the NHX (Na+/H+ exchanger) family. Moreover, we review the functional role of the tonoplast-specific phosphoinositide PI(3,5)P2, able to simultaneously inhibit the activity of NHXs and CLC-a transporters, whose coordinated action can play an important role in the water balance of plant cells.

Publisher

Walter de Gruyter GmbH

Subject

Cellular and Molecular Neuroscience,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://www.degruyter.com/document/doi/10.1515/bmc-2022-0009/pdf

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