Bestimmung partieller photochemisdier Quantenausbeuten iiber kombinierte Absorptions- und Fluoreszenzmessung / On the Determination of Partial Photochemical Quantum Yields by Combined Absorbance and Fluorescence Measurement

Abstract

A combined automatic set up for the measurement of fluorescence and absorbance is described. The absorbance at irradiation wavelength E' and a fluorescence reaction spectrum are measured continously and simultaneously. Even for solutions with high concentrations up to E'>1, the fluorescence intensity can be corrected for. By this method the photodegradation of fluorescent dyes was examined. Because only a few of the degradation products fluoresce, the determination of kinetic parameters is easier than from absorbance measurements. The method gave good results for laser dyes using even high concentrations (E'> 1), with less calculatory expenditure and smaller standard deviation in comparison to the evaluation of absorbance measurements only