Functional analysis of sepiapterin reductase in Drosophila melanogaster-Reference-Cited by-同舟云学术

Functional analysis of sepiapterin reductase in Drosophila melanogaster

Published:2015-05-06 Issue:2 Volume:26 Page:63-68
ISSN:2195-4720
Container-title:Pteridines
language:en
Short-container-title:

Author:

Kim Kiyoung¹,Kim Heuijong²,Yim Jeongbin¹

Affiliation:

1. Department of Medical Biotechnology, Soonchunhyang University, Asan 336-745, Korea

2. School of Biological Sciences, Seoul National University, Seoul 151-742, Korea

Abstract

Abstract Sepiapterin reductase (SR) is a key enzyme involved in the biosynthesis of tetrahydrobiopterin (BH4), an essential cofactor for the synthesis of important biogenic amines, including catecholamines and serotonin. BH4 deficiencies have been implicated in several neurological disorders. Here, we characterized sepiapterin reductase (SR) loss-of-function mutants in Drosophila melanogaster and demonstrated that SR mutations are responsible for hyposensitivity to oxidative stress. Biochemical analysis further revealed that SR activity and BH4 levels in SR mutants were significantly reduced. Furthermore, we showed that the levels of phosphorylated Akt and total Akt protein were increased in SR mutants. Our findings indicate that SR plays an important role in the Akt pathway and that SR mutants will be a valuable tool for investigating the physiological functions of BH4.

Publisher

Walter de Gruyter GmbH

Subject

Clinical Biochemistry,Molecular Medicine,Biochemistry

Link

https://www.degruyter.com/document/doi/10.1515/pterid-2014-0018/pdf

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