Digital Computer Analysis of Electrophoresis and Ultracentrifugation Patterns by a Sum of Gaussian-like Distributions

Abstract

Abstract Computer Analysis, Electrophoresis, Ultracentrifugation A Gaussian-like distribution function with three additional parameters is introduced which describes well the electrophoresis patterns of albumin, prealbumin and transferrin. Electrophoresis and ultracentrifugation patterns with 10-15 overlapping peaks are analyzed by means of the FORTRAN-program DIANA. One obtains the (relative) areas, positions and widths of the peaks. The analysis of a series of patterns proceeds in an automated way after the number of molecular components as well as starting values of the positions and the widths have been chosen in a test period.