CD45RO regulates the HIV-1 gp120-mediated apoptosis of T cells by activating Lck-Reference-Cited by-同舟云学术

CD45RO regulates the HIV-1 gp120-mediated apoptosis of T cells by activating Lck

Published:2018-03-17 Issue:6 Volume:399 Page:583-591
ISSN:1437-4315
Container-title:Biological Chemistry
language:en
Short-container-title:

Author:

Li Kelei¹²³,Cong Zhe¹²³,Peng Zhuoying¹²³,Chen Ting¹²³,Xue Jing¹²³,Wei Qiang¹²³

Affiliation:

1. Institute of Laboratory Animal Science , Chinese Academy of Medical Sciences (CAMS) , No. 5 Panjiayuan Nanli, Chaoyang District , Beijing 100021 , China

2. Comparative Medicine Center, Peking Union Medical College (PUMC) , Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases , Beijing , China

3. Center for AIDS Research, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing , China

Abstract

Abstract CD45 has been reported to regulate the HIV-1 gp120-induced apoptosis of Jurkat cells. Here, we demonstrate that the extracellular domain of CD45 plays an important role in this function. We observed that CD45RO-transfected cells, but not cells transfected with other CD45 isoforms, underwent significant apoptosis induced by gp120. However, a CD45RA-transfected cell line treated with an O-glycan inhibitor was able to undergo apoptosis. The role of the extracellular domain of CD45 was further confirmed using CD45 isoform-transfected cell lines by analyzing the phosphorylation of Lck, which is a direct substrate of CD45 tyrosine phosphatase, and by using an Lck inhibitor. These results suggest that CD45RO modulates HIV-1 gp120-induced apoptosis by regulating the activity of Lck.

Publisher

Walter de Gruyter GmbH

Subject

Clinical Biochemistry,Molecular Biology,Biochemistry

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