Metabolie Activity of Hydroxycinnamic Acid Glucose Esters in Cell Suspension Cultures of Chenopodium rubrum

Abstract

Abstract Cell suspension cultures of Chenopodium rubrum accumulate high amounts of metabolically active glucose esters of p-coumaric and ferulic acid. Pulse-labelling experiments using [14C]phenylalanine and application of the phenylalanine ammonia-lyase (PAL) inhibitor L-α-aminooxy-β-pnenylpropionic acid (AOPP) revealed that the glucose esters are subject to high turnover. A considerable portion of the ferulic acid was found as an insoluble component, probably bound to cell wall material. Application of m-fluoro-DL-tyrosine (MFT), an effective inhibitor of tyrosine biosynthesis via L-arogenate, markedly increased the amount of 1-p-coumaroyl-and 1-feruloyl-glucose accumulated.