Author:
Nemescu Roxana Elena,Ursu Ramona Gabriela,Dorobăț Carmen Mihaela,Iancu Luminița Smaranda
Abstract
AbstractMeningococcal infection requires a fast and accurate diagnostic method in order to correctly initiate the antibiotic therapy. The aim of our study was to assess the efficiency of Real Time PCR -Taq Man using sod C gene / N. meningitidis in comparison with the classical methods for the diagnosis of meningococcal infection - direct microscopy, cultivation, latex agglutination and blood culture. We have detected 24/44 (54.54%) patients with meningococcal infection. In both cases of patients with / without previous antibiotic therapy before admission, the AUC (area under curve) had the highest values for RT PCR in CSF and blood analysis. This sod C RT-PCR assay is a highly sensitive and specific method for detection of Neisseria meningitis and it would be useful to include this method like a multiplex in routine testing of patients with clinical meningococcal infection for other etiological agents also.
Reference46 articles.
1. Detection of acute childhood meningitis by culture and agglutination tests in Tabriz Iran Iran;Ghotaslou;Acta Med,2012
2. Early treatment with parenteral penicillin in meningococcal disease Jul;Cartwright,1992
3. Conquering the meningococcus Jan;Stephens;FEMS Microbiol Rev,2007
4. The guidelines minimum information for publication of quantitative real - time PCR experiments;Bustin;Clin Chem,2009
5. Distribution of invasive meningococcal disease in Italian pediatric population : implications for vaccination timing Feb;Azzari;Vaccine,2014
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献