Author:
Khosa Sakshi,AlKhatib Zainab,Smits Sander H.J.
Abstract
Abstract
Nisin is a lantibiotic produced by Lactococcus lactis (L. lactis), which is active against many Gram-positive bacteria. However, in various pathogenic and nonpathogenic bacteria, the presence of a nisin resistance protein (NSR) confers resistance against nisin. Here, we show that NSR from Streptococcus agalactiae (SaNSR) confers 20-fold resistance when expressed in L. lactis. We also show that SaNSR is encoded by an operon structure comprising of a lipoprotein and an ATP-binding cassette transporter as well as a two-component system that is putatively involved in expression and regulation. This organization of the operon is conserved in several (non)pathogenic strains that do not produce nisin themselves.
Subject
Clinical Biochemistry,Molecular Biology,Biochemistry
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