Author:
Seiz Lina,Kotzsch Matthias,Grebenchtchikov Nicolai I.,Geurts-Moespot Anneke J.,Fuessel Susanne,Goettig Peter,Gkazepis Apostolos,Wirth Manfred P.,Schmitt Manfred,Lossnitzer Arndt,Sweep Fred C.G.J.,Magdolen Viktor
Abstract
AbstractKLK4 is a member of the human kallikrein-related peptidase family of (chymo)trypsin-like serine proteases. The aim of the present study was to generate polyclonal antibodies (pAb) directed against KLK4 for the analysis of KLK4 by immunohistochemistry in human tissues. Recombinantly expressed human mature KLK4 was used for immunization of chickens. pAb 617A is an affinity-purified monospecific pAb fraction reacting with a linear epitope within a flexible surface-exposed loop of KLK4. pAb 617C is the KLK-directed pAb fraction completely depleted from pAb 617A. In healthy adult tissues, KLK4 was immunodetected by both antibody fractions in kidney, liver, and prostate, but not in other organs such as colon and lung. To evaluate protein expression of KLK4 in prostate cancer, samples of tumor tissue plus corresponding tumor-free areas of 44 prostate cancer patients, represented on a tissue microarray, were investigated. Distinct KLK4 immunostaining was observed with both antibodies in cancerous glandular epithelial cells, but not in surrounding stromal cells. KLK4 expression was lower in stage pT3+4 than in pT1+2 tumors, which was highly significant when employing pAb 617A. Thus, our results indicate that KLK4, which is expressed in the healthy prostate, is upregulated in early-stage but not late-stage prostate cancer.
Subject
Clinical Biochemistry,Molecular Biology,Biochemistry
Reference24 articles.
1. Direct progesterone receptor and indirect androgen receptor interactions with the kallikrein - related peptidase gene promoter in breast and prostate cancer;Lai;Mol Cancer Res,2009
2. Kallikrein - related pep - tidase initiates intracellular signaling via protease - acti - vated receptors KLK and PAR - are co - expressed during prostate cancer progression;Ramsay;Biol Chem,2008
3. Kallikrein is a proliferative factor that is overexpressed in prostate cancer;Klokk;Cancer Res,2007
4. Structures and specificity of the human kallikrein - related peptidases KLK;Debela;Biol Chem,2008
5. Specific increase of human kallikrein mRNA and protein levels in breast cancer stromal cells Bio phys;Mange;Biochem Res Commun,2008
Cited by
33 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献