The role of GFAP and vimentin in learning and memory
Author:
Wilhelmsson Ulrika1, Pozo-Rodrigalvarez Andrea2, Kalm Marie3, de Pablo Yolanda1, Widestrand Åsa1, Pekna Marcela245, Pekny Milos145
Affiliation:
1. Laboratory of Astrocyte Biology and CNS Regeneration, Center for Brain Repair , Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg , Box 440 , S-40530 Gothenburg , Sweden 2. Laboratory of Regenerative Neuroimmunology, Center for Brain Repair , Department of Clinical Neuroscience, Institute of Neuroscience and Physiology , Sahlgrenska Academy at the University of Gothenburg , S-40530 Gothenburg , Sweden 3. Department of Pharmacology, Institute of Neuroscience and Physiology , Sahlgrenska Academy at the University of Gothenburg , S-40530 Gothenburg , Sweden 4. Florey Institute of Neuroscience and Mental Health , Parkville, VIC , Australia 5. University of Newcastle , Newcastle , NSW , Australia
Abstract
Abstract
Intermediate filaments (also termed nanofilaments) are involved in many cellular functions and play important roles in cellular responses to stress. The upregulation of glial fibrillary acidic protein (GFAP) and vimentin (Vim), intermediate filament proteins of astrocytes, is the hallmark of astrocyte activation and reactive gliosis in response to injury, ischemia or neurodegeneration. Reactive gliosis is essential for the protective role of astrocytes at acute stages of neurotrauma or ischemic stroke. However, GFAP and Vim were also linked to neural plasticity and regenerative responses in healthy and injured brain. Mice deficient for GFAP and vimentin (GFAP
−/−
Vim
−/−) exhibit increased post-traumatic synaptic plasticity and increased basal and post-traumatic hippocampal neurogenesis. Here we assessed the locomotor and exploratory behavior of GFAP
−/−
Vim
−/− mice, their learning, memory and memory extinction, by using the open field, object recognition and Morris water maze tests, trace fear conditioning, and by recording reversal learning in IntelliCages. While the locomotion, exploratory behavior and learning of GFAP
−/−
Vim
−/− mice, as assessed by object recognition, the Morris water maze, and trace fear conditioning tests, were comparable to wildtype mice, GFAP
−/−
Vim
−/− mice showed more pronounced memory extinction when tested in IntelliCages, a finding compatible with the scenario of an increased rate of reorganization of the hippocampal circuitry.
Publisher
Walter de Gruyter GmbH
Subject
Clinical Biochemistry,Molecular Biology,Biochemistry
Reference76 articles.
1. Akers, K.G., Martinez-Canabal, A., Restivo, L., Yiu, A.P., De Cristofaro, A., Hsiang, H.L., Wheeler, A.L., Guskjolen, A., Niibori, Y., Shoji, H., et al. (2014). Hippocampal neurogenesis regulates forgetting during adulthood and infancy. Science 344, 598–602. 2. Allen, N.J., Bennett, M.L., Foo, L.C., Wang, G.X., Chakraborty, C., Smith, S.J., and Barres, B.A. (2012). Astrocyte glypicans 4 and 6 promote formation of excitatory synapses via GluA1 AMPA receptors. Nature 486, 410–414. 3. Anacker, C. and Hen, R. (2017). Adult hippocampal neurogenesis and cognitive flexibility – linking memory and mood. Nat. Rev. Neurosci. 18, 335–346. 4. Berg, A., Zelano, J., Pekna, M., Wilhelmsson, U., Pekny, M., and Cullheim, S. (2013). Axonal regeneration after sciatic nerve lesion is delayed but complete in GFAP- and vimentin-deficient mice. PLoS One 8, e79395. 5. Bevins, R.A. and Besheer, J. (2006). Object recognition in rats and mice: a one-trial non-matching-to-sample learning task to study ‘recognition memory’. Nat. Protoc. 1, 1306–1311.
Cited by
46 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|