Affiliation:
1. Department of Chemistry , Women University Swabi , Swabi , Pakistan
2. Department of Biochemistry , Women University Swabi , Swabi , Pakistan
Abstract
Abstract
Objectives
Microorganisms produce optically single bioactive natural products; the process is cheap as compared to chemical synthesis and environmentally friendly. Geodin 1 was isolated from the soft coral-derived fungus Aspergillus sp. It has a broad range of biological activities such as antiviral, antimicrobial, glucose stimulator for rat adipocytes, enhancement of fibrinolytic and cytotoxic activities, and is a subunit of the first nonpeptide and inhibitory active galanin receptor. In this work, we studied the effects of sodium chloride (NaCl) salt concentration on the production of geodin 1 and improved its yield to a multi-gram quantity through media optimization from a marine-derived fungus Aspergillus sp.
Methods
The fungal strain was cultivated at various concentrations of NaCl salt in rice medium and extracted after different intervals of time.
Results
The yield of geodin 1 was 137.2 mg/L at optimal conditions. The optimal conditions for the high yield of geodin 1 were found as rice medium with 2.0% NaCl salt and 3 weeks incubation at room temperature.
Conclusions
The concentration of NaCl salt greatly affects the yield of geodin and hence its biosynthetic pathway.
Funder
Program of the National Natural Science Foundation of China
the Fundamental Research Funds for the Central Universities
Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University
the Project of State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources
Ministry of Education of China
Subject
Biochemistry (medical),Clinical Biochemistry,Molecular Biology,Biochemistry
Cited by
1 articles.
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