Identification and Quantitation of Usnic Acid from the Lichen Usnea Species of Anatolia and Antimicrobial Activity

Author:

Cansaran Demet1,Kahya Didem2,Yurdakulol Ender1,Atakol Orhan2

Affiliation:

1. Department of Biology, Botany Section, Faculty of Science, University of Ankara, 06100, Tandogan, Ankara, Turkey. Fax: +90-3122232395.

2. Department of Chemistry, Faculty of Science, University of Ankara, 06100, Tandogan, Ankara, Turkey

Abstract

Abstract Six species of lichens, such as Usnea florida, Usnea barbata, Usnea longissima, Usnea rigida, Usnea hirta and Usnea subflorida, were collected from different areas of Anatolia (district of Antalya, Karabük, Çankırı, Giresun and Trabzon) in Turkey. Their usnic acid amounts in acetone extracts were determined by HPLC. In addition, antimicrobial activities of these extracts were determined against Escherichia coli (ATCC 35218), Enterococcus faecalis (RSKK 508), Proteus mirabilis (Pasteur Ens. 235), Staphylococcus aureus, Bacillus subtilis and Bacillus megaterium. It was shown that with increasing amount of usnic acid, the antimicrobial activity increased. Usnic acid contents of Usnea species varied between 0.22-6.49% of dry weight.

Publisher

Walter de Gruyter GmbH

Subject

General Biochemistry, Genetics and Molecular Biology

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