Isolation and HPLC Quantitative Analysis of Antioxidant Flavonoids from Alternanthera tenella Colla-Reference-Cited by-同舟云学术

Isolation and HPLC Quantitative Analysis of Antioxidant Flavonoids from Alternanthera tenella Colla

Published:2006-02-01 Issue:1-2 Volume:61 Page:19-25
ISSN:1865-7125
Container-title:Zeitschrift für Naturforschung C
language:en
Short-container-title:

Author:

Salvador Marcos J.¹²³⁴,Ferreira Eliane O.²⁵,Mertens-Talcott Susanne U.²,De Castro Whocely Victor²,Butterweck Veronika²,Derendorf Hartmut²,Dias Diones A.⁵

Affiliation:

1. Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeiraõ Preto, Universidade de Saõ Paulo, Avenida dos Bandeirantes, 3900, 14040-901, Ribeiraõ Preto, Saõ Paulo, Brasil

2. Department of Pharmaceutics, College of Pharmacy, University of Florida, PO Box 100494, Gainesville FL 32610-0494, USA

3. Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeiraõ Preto, Universidade de Saõ Paulo, Via do café, s/n, 14040-903, Ribeiraõ Preto, Saõ Paulo, Brasil.

4. Current address: Instituto de Pesquisa

5. Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeiraõ Preto, Universidade de Saõ Paulo, Via do café, s/n, 14040-903, Ribeiraõ Preto, Saõ Paulo, Brasil

Abstract

Abstract Phytochemical analysis of the antioxidant ethanolic extract of Alternanthera tenella Colla led to the isolation of six flavonoids, acacetin 8-C-[α-ʟ-rhamnopyranosyl-(1→2)-β-ᴅ-glucopyranoside] (1), 2″-O-α-ʟ-rhamnopyranosyl-vitexin (2), 2″-O-β-d-glucopyranosyl-vitexin (3), vitexin (4), quercetin (5) and kaempferol (6). All the structures were established by ESIMS and NMR spectroscopic methods. Antioxidant capacity of extract, fractions and isolated compounds was determined using the oxygen radical absorbance capacity (ORAC) assay and extract, fractions and flavonoids isolated showed antioxidant activity in vitro. Moreover, the total soluble phenolic contents of the extract and fractions were measured using the Folin- Ciocalteau reagent and the quantitative analysis of flavone C-glycosides major constituents was performed by HPLC

Publisher

Walter de Gruyter GmbH

Subject

General Biochemistry, Genetics and Molecular Biology

Link

https://www.degruyter.com/document/doi/10.1515/znc-2006-1-204/pdf

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