Spurenanalytik von p-Benzochinon-und Hydrochinon-Derivaten mit Gaschromatographie und Gaschromatographie/Massenspektrometrie. Identifizierung von Wehrsekret-Komponenten europäischer Juliden / Trace Analysis of p-Benzoquinone-and Hydroquinone Derivatives by Gas-Liquid Chromatography and Gas-Liquid Chromatography/Mass-Spectrometry. Identification of Defensive Secretion Components from European Julids

Abstract

Substituted p-benzoquinones (= quinones) and substituted hydroquinones [bis-trimethylsilyl- ethers or bis-trifluoroacetates] can be separated by gas-liquid chromatography using 50 meter long thinfilm capillaries (inner diameter: 0.25 mm) coated with silicone OV 17 (50% phenyl, methyl) or silicone DC 550 (25% phenyl, methyl). Total retention times of various derivatives of p-benzoquinone and hydroquinone were deter­mined. Complex model mixtures of these derivatives can be separated by gas-liquid chromatography with temperature programs and the individual components can be clearly identified by their mass spectra in the sub microgram range by using combined gas-liquid chromatography/mass-spectrometry. This microanalytical technique was applied to identify the defensive secretion components from members of the diplopod order Julida (Arthropods). Julus nitidus produces a defensive secretion that consists exclusively of 2-methyl-3-methoxyquinone. The expelled defensive secretions of Unciger foetidus, Cylindroiulus coeruleocinctus (Iondinensis), Cylindroiulus punctatus, Cylindroiulus luridus and Ophyiulus psilosus consist of a 2-methylquinone and 2-methyl-3-methoxyquinone mixture. The presence of the corresponding hydroquinones-2-methyl-hydroquinone and 2-methyl-3-methoxyhydroquinone-was also determined in all cases.