Affiliation:
1. Federal Research Institute for Animal Virus Diseases, Tübingen
Abstract
Abstract
Hog cholera virus grown in PK-15 cells and SK-cells was labeled with [35S] methionine and [3H] uridine. At least 3 polypeptides were resolved by polyacrylamide gel electro phoresis after disruption of the virus with sodium dodecyl-sulfate. The molecular weights of the structural proteins were determined to be 55000 (p55), 46000 (gp46), and 36000 (p36). The molecular weight of the viral RNA was determined to be about 4 X 106 in polyacrylamide-agarose-gel electrophoresis. In sucrose gradients the RNA has a S20,w value of 40 -45S.
Subject
General Biochemistry, Genetics and Molecular Biology
Cited by
10 articles.
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