Prevalence and some virulence genes of Escherichia coli O157 isolated from chicken meats and giblets-Reference-Cited by-同舟云学术

Prevalence and some virulence genes of Escherichia coli O157 isolated from chicken meats and giblets

Published:2017-04-28 Issue:2 Volume:17 Page:555-563
ISSN:2300-8733
Container-title:Annals of Animal Science
language:en
Short-container-title:

Author:

Guran Husnu Sahan¹,Vural Aydın¹,Erkan Mehmet Emin¹,Durmusoglu Halil¹

Affiliation:

1. Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Dicle University, 21280, Diyarbakir, Turkey

Abstract

Abstract Escherichia coli O157 related foodborne illnesses continue to be one of the most important global public health problems in the world. This study aims to determine E. coli O157 prevalence in 375 chicken meat parts and giblets. The samples were collected randomly from several supermarkets and butchers in Diyarbakir, a city in southeast Turkey. They were analyzed and confirmed using the immunomagnetic separation (IMS), Vitek® 2 microbial identification system and polymerase chain reaction (PCR) method. This study also aims to detect the presence of fliCH7, eaeA, stx1, stx2 and hlyA genes by using PCR. The overall E. coli O157 prevalence in chicken meat parts and giblets was 1.3%. All of the E. coli O157 isolates carried rfbEO157 and eaeA genes; but not any fliCH7 and hlyA genes. The E. coli O157 isolates obtained from drumstick and breast meat carried either stx1 or stx2 genes, which were related to important virulence factors of the disease.

Publisher

Walter de Gruyter GmbH

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