Production and characterization of a bioflocculant produced by Proteus mirabilis AB 932526.1 and its application in wastewater treatment and dye removal

Abstract

Abstract Microbial flocculants affect the aggregation of suspended solutes in solutions, thus, they are a viable alternative to inorganic and organic synthetic flocculants which are associated with deleterious health problems. Moreover, a potential solution for wastewater treatment. The study aimed to produce and characterize a bioflocculant from Proteus mirabilis AB 932526.1 and apply it in domestic wastewater treatment and dye removal. The bioflocculant was extracted using butanol and chloroform (5:2 v/v). Carbohydrates, proteins, and uronic acid were identified using phenol-sulphuric acid, Bradford, and Carbazole essays. The morphology, crystallinity and elemental composition of the purified bioflocculant were determined using a Scanning electron microscope (SEM), X-ray diffraction analysis and SEM energy dispersive elemental detector (SEM-EDX). The antimicrobial properties and dye removal efficiencies were evaluated. About 3.8 g/L yields of the purified bioflocculant were attained. Chemical composition analysis revealed the presence of 65 % carbohydrates, 10 % proteins, and 24 % uronic acids. The bioflocculant displayed an amorphous and crystalline structure. Bioflocculant further shows some remarkable properties as they can be able to inhibit the growth of both Gram-positive and Gram-negative microorganisms. The removal efficiencies of 85 % (COD), 82 % (BOD), and 81 % (SO4 2−) in domestic wastewater were achieved. Moreover, the high removal efficiency of staining dyes such as methylene blue (71 %), carbol fuchsin (81 %), safranin (83 %), methylene orange (90 %), and Congo red (90 %) were found. The produced bioflocculant can imply industrial applicability.