Abhängigkeit des Teilungswachstums isolierter Einzelzellen aus Vitis vinifera-Kulturen von benachbarten wachsenden Geweben

Abstract

By means of a new plating technique, single isolated cells from tissue cultures of Vitis vinifera could be grown with a plating efficiency between 60 and 70 percent. Division of these cells occurred only in the presence of living tissue fragments and cell groups. It is shown quantitatively that the rate as well as the time of division of these cells is determined by the weight of the inoculum. Some aspects of these findings and additional observations are discussed.