Inaktivierungsversuche mit homozygoten Hefestämmen verschiedenen Ploidiegrades

Abstract

A diploid Saccaromyces strain was treated with several doses of X-rays, UV and succinic acid peroxyde (BPO). The inactivation of the ability to form macroscopic colonies as well as the ability to form microcolonies of at least two cells to a few hundred cells has been compared with the inactivation of respiration and fermentation intensity. If the inactivation of macroscopic colony formation is taken as a measure of the effective dose applied, the formation of at least one daughter cell as well as respiration and fermentation intensity is reduced to approximately the same extent after BPO and X-ray treatment. In the latter case, however, much higher effective doses have to be applied and a distinct difference between respiration and fermentation sensitivity is observed. After UV-treatment the formation of at least one daughter cell is exceedingly more sensitive than the fermentation processes. The respiration processes behave most UV resistant. Possible reasons for the observed different relative sensitivities are discussed.